Modifying Enzymes
Sigma Aldrich Fine Chemicals Biosciences Papain from papaya latex buffered aqueous suspension, 2× Crystallized, ≥16 units/mg protein | 9001-73-4 | MFCD00131791 | 25mg
Papain from papaya latex buffered aqueous suspension, 2× Crystallized, ≥16 units/mg protein | 9001-73-4 | MFCD00131791 | 25mg
As One International Inc Betaine Enhancer solution 5 M, 5 x 1 ml
Betaine Enhancer Solution is an excellent additive over a wide range of different templates, including GC-rich sequences and templates with a high degree of secondary structures. Betaine binds preferentially to AT rich sequences in the major groove, thereby stabilizing AT rich regions of the DNA. Because AT forms 2 hydrogen bonds and GC forms 3, the bonding of AT is less stable than the one of GC. As a consequence of the stabilizing effect of Betaine on AT bonding, the stability of AT bonding and GC bonding is brought close to an equal level. At the same time, Betaine has a sequence independent destabilizing effect on all DNA. The Tm of AT rich and GC rich sequences are equalized and the overall Tm is lowered. Furthermore, Betaine aids the processivity of thermostable polymerases and reduces "pauses" in polymerization caused by secondary structure that can induce the polymerase to disassociate from the DNA strand.
Sino Biological Recombinant DNase I (RNase-free) 1000 U
DNase I is an endonuclease that can non-specifically cleave DNA, producing dinucleotide, trinucleotide, and oligonucleotide products with 5 ́ phosphorylated and 3 ́ hydroxylated ends. DNase I acts on single-and double-stranded DNA, chromatin, and RNA:DNA hybrid strands. Certified by highly sensitive fluorescence probe assays, our DNase I (RNase-free) demonstrates virtually undetectable RNase activity-a level of purity that surpasses other commercial sources. Trust in this "Ultra-Pure" standard for the ultimate reliability in your RNase-sensitive workflows.
Carnabio Usa Inc ROCK1/10UG/CATALYTIC
ROCK1, Catalytic domain, Wild type, Amino Acid 1-477, NP_005397.1, Expressed in Insect (sf21); 10microg
Worthington Biochemical Corporation PAPAIN LYOPHILIZED 1GM
Supplied as a lyophilized powder prepared from a 2X crystalline suspension, Code: PAP. To insure full activity, the enzyme should be incubated in a solution containing 1.1 mM EDTA, 0.067mM mercaptoethanol and 5.5 mM cysteine-HCl for 30 minutes. It is recommended that the enzyme be 0.22 micron filtered after dissolution and prior to use. 9001-73-4
Worthington Biochemical Corporation ELASTASE LYOPHILIZED 1GM
Two times crystallized, (Code: ESL), supplied as a dialyzed, lyophilized powder. The enzyme should be 0.22 micron filtered after reconstitution and prior to use. Suitable for the isolation of Type II lung cells. 2594-83-7
Apexbio Technology LLC EZ Cap OVA mRNA (5-moUTP) 5x1mg (1mg/mL)
Ovalbumin (OVA) mRNA encodes a egg white glycoprotein with a molecular weight of approximately 45 000 daltons It can induce moderate immunogenicity in organisms and is commonly used as an immunogen in immunological experiments such as establishing animal models for hypersensitivity asthma etc EZ Cap OVA mRNA (5-moUTP) is provided at 1 mg/mL capped by co-transcriptional capping with a Cap 1 structure and polyadenylated Modified 5-moUTP incorporation reduces the host cell immune response and enhances mRNA stability and translation efficiency
Electron Microscopy Sciences Trypsin 1:100, Powder 25 GR
F.W. 372.09
Extract from Porcine Pancreas. Used for whole cell preparations. Activity >100 NF units/mg.
Sigma Aldrich Fine Chemicals Biosciences Proteinase K from Tritirachium album ≥3.0 unit/mg solid, lyophilized powder | 39450-01-6 | MFCD00132129 250mg
Proteinase K from Tritirachium album ≥3.0 unit/mg solid, lyophilized powder | 39450-01-6 | MFCD00132129 | 250mg
Bioassay Systems QuantiChrom™ Pectinase Assay Kit. For quantitative determination of pectinase activity determination in biological samples.
For quantitative determination of pectinase activity determination in biological samples. Key Features: Linear detection range from 9.2-100 U/L pectinase activity in a 96-well plate assay. Simple and convenient 40 minute "Add-Mix-Measure" type assay. High-throughput format and compatible with laboratory liquid handling systems. No heating required. The kit does not use toxic materials. Refer to the previously established DNS method. Method: OD600nm. Samples: Enzyme extracts, agriculture and biological samples. Species: Plant, fungal tissues, juice, bacteria, etc. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 9.2 U/L.
New England Biolabs, Inc. mRNA Decapping Enzyme – 2000 units
mRNA Decapping Enzyme catalyzes the removal of 7-methylguanosine cap (m7G) from 5 end of mRNA, producing 5 monophosphate and releasing m7GDP. mRNA Decapping Enzyme is capable of decapping mRNAs of various lengths and removes both Cap0 and Cap1 structures with similar efficiency. mRNA Decapping Enzyme also converts 5 triphosphate ends to 5 monophosphate, albeit with reduced efficiency. 5' monophosphorylated RNA can be exploited in a variety of downstream applications, including 5' RNA Ligase-mediated RACE, RNA-seq, and 5' -> 3' exonuclease digestion.
Sigma Aldrich Fine Chemicals Biosciences N Glycosidase f recombinan
Peptide-N-glycosidase f Peptide-N4-(acetyl-beta-glucosaminyl)-asparagine amidase
New England Biolabs, Inc. Thermostable Inorganic Pyrophosphatase – 250 units
Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate: P2O7-4 + H2O -> 2HPO4-2.
Qiagen Beverly Inc T4 DNA LIGASE
DNA Ligase; T4 DNA Ligase; Conc. 120,000 U/ml; 150,000 U; incl. 10X buffer
Bioworld Cellulase (Onozuka R-10), 5 g
Cellulase (Onozuka R-10) From Trichadema Viride. Enzyme mixture often used in combination with Macerozyme R-10 (M22010) for isolation of plant protoplasts. One unit of Cellulase will liberate 1.0 µmole of glucose from carboxymethyl cellulose.Optimum pH range: 4-5.Soluble in water (10mg/ml)Contents:Cellulase: 1.0 U/mg Pectinase: 0.4 U/mg Hemicellulase: 1.0 U/mg alpha-Amylase: 0.6 U/mg Protease: 0.01 U/mg