Modifying Enzymes
Apexbio Technology LLC DNase I (RNase-free) 10000U
DNase I (RNase-free) is an endodeoxyribonuclease capable of hydrolyzing single-stranded or double-stranded DNA producing dinucleotide trinucleotide and oligonucleotide fragments with 3 -OH and 5 -phosphate ends The activity of DNase I (RNase-free) depends on Ca2 and can be activated by Mg2 or Mn2 In the presence of Mg2 DNase I (RNase-free) randomly cleaves double-stranded DNA at any site in the presence of Mn2 it simultaneously recognizes both DNA strands and cleaves at nearly identical sites DNase I (RNase-free) acts on single-stranded DNA double-stranded DNA chromatin and RNA DNA hybrids In molecular biology experiments DNase I (RNase-free) is suitable for the removal of DNA in RNA extraction in vitro transcription and RT-PCR applications
Cell Signaling Technology Deoxyribonuclease I Recombina
Deoxyribonuclease I, Recombinant (Animal-Free, Protease and RNase Free) 1 vial
New England Biolabs, Inc. Tte-UvrD Helicase – 0.5 µg
Tte UvrD Helicase is a repair helicase capable of unwinding double-stranded DNA, without a requirement for a specific flap or overhang structure, from the thermophilic organism Thermoanaerobacter tengcongensis. It is active on a wide range of DNA substrates and, along with its thermostability (active to 70C), Tte UvrD Helicase has been demonstrated to be a useful additive for improving specificity of isothermal amplification reactions, particularly in conjunction with the WarmStart LAMP Kit.
New England Biolabs, Inc. T4 Polynucleotide Kinase Reaction Buffer – 4 ml
New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of the enzyme.
Apexbio Technology LLC DNase I (RNase-free),1000U
DNase I (RNase-free) is an endonuclease that digests single- or double- stranded DNA, generating dinucleotide, trinucleotide, and oligonucleotide products with 5´-phosphorylated and 3´-hydroxylated ends. The activity of DNase I (RNase-free) dependent on Ca2+ and can be activated by Mg2+ or Mn2+. In the presence of Mg2+, DNase I (RNase-free) can randomly cleaves arbitrary sites of double-stranded DNA; in the presence of Mn2+, DNase I (RNase-free) can simultaneously recognize both strands of DNA and cleaves at nearly same sites. DNase I (RNase-free) can act on single stranded DNA, double stranded DNA, chromatin, and RNA: DNA hybrid strands. DNase I (RNase-free) is suitable for RNA extraction, in vitro transcription, and removal of DNA for RT-PCR experiments.Other sizes are also available. Please inqury us for quote.
Cayman Chemical ANTIBODY KU-0058948 10mg
A PARP1 and PARP2 inhibitor (IC50s = 3.4 and 1.5 nM, respectively); selective for PARP1 over PARP3, PARP4, and tankyrase (IC50s = 40, 1,200, and >10,000 nM, respectively); selectively inhibits the survival of embryonic stem cells lacking Brca1 or Brca2 over embryonic stem cells expressing wild-type Brca1 or Brca2; decreases the survival and induces apoptosis in P39 and MUTZ-3 AML cells at 1 µM; potentiates MS-275-induced cytotoxicity in patient-derived AML cells at 5 nM; reduces survival in PTEN-deficient HCT116 cells but not those expressing wild-type PTEN or HCT116+/neo
Carnabio Usa Inc BTN - TGFBR1(TGFBR1) / 10UG
BTN-TGFR1(TGFBR1), Catalytic domain, Wild type, Amino Acid 200-503(end), NP_004603.1, Expressed in Insect (sf21); 10microg
New England Biolabs, Inc. Taq DNA Ligase – 10000 units
Taq DNA Ligase is a thermostable ligase that catalyzes the formation of a phosphodiester bond between the 5'-phosphate and the 3'-hydroxyl of two adjacent DNA strands. The strands to be ligated need to be hybridized and accurately paired, with no gap, to a complementary DNA strand; allowing resolution of single nucleotide variants. Taq DNA Ligase uses NAD as a cofactor and it is active at elevated temperatures (37 C - 75 C).
New England Biolabs, Inc. Immobilized T4 DNA Ligase - 1.1 mg
Immobilized T4 DNA Ligase (IM-T4 DNA Ligase) is a slurry of magnetic beads coated with T4 DNA Ligase to produce a 10 mg/ml solution (50% glycerol) with an effective concentration of 60 cohesive end units (CEU) per microliter of slurry. Following a reaction, enzyme can be removed using a magnet thereby avoiding heat inactivation, and can be re-used.
Enzo Life Sciences MG-132 (5 mg) CAS: 133407-82-6
Key inhibitor for use in proteasome research. Alternative Name: Z-LLL-CHO. Formula: C26H41N3O5. MW: 475.6. CAS: 133407-82-6. Purity: ≥98%. Sequence Z-Leu-Leu-Leu-CHO. Appearance: White solid. Solubility: Soluble in DMSO (25mg/ml) or 100% ethanol (25mg/ml). Long Term Storage: -80°C. Use/Stability: Solutions are stable for up to one week if stored at -20°C. Solutions are stable for up to two months if stored at -80°C.
Diagenode Inc Hyperactive Tn5 transposase, Unloaded, 20 μl, Mg++ dependent, pH 7.5-8.0, 37-55°C
Hyperactive Tn5 transposase, Unloaded, 20 μl, Mg++ dependent, pH 7.5-8.0, 37-55°C
ACROBiosystems The enzyme hydrolyzes the β-1,4 linkage between the core GlcNAc residues in the Fc-glycan, leaving the innermost GlcNAc intact on the Fc. It is derived from Streptococcus pyogenes and expressed in E. coli. The enzyme contains a His-tag and
EndoS is an endoglycosidase from Streptococcus pyogenes that specifically hydrolyzes N-linked glycans in the Fc-region of native IgG. It specifically deglycosylates the conserved N-glycans on the Fc region of IgG by hydrolyzing the chitobiose core (at the beta -1,4 linkage between the two N-acetylglucosamines) of the N-glycans, cleavage leave one GlcNAc residue remaining attached to the asparagine residue on the peptide backbone.
Supply Solutions Roche - Nuclease S7; Micrococcal nuclease, from Staphylococcus aureus; pkg of 15,000 U
Roche - Nuclease S7; Micrococcal nuclease, from Staphylococcus aureus; pkg of 15,000 U